|Run||Spots||Bases||Size||GC content||Published||Access Type|
This run has 1 read per spot:
Technical read Application Read L=4, 100% Length is 4, 100% spots contain this read ̅L=165, σ=92.8, 66% Average length is 165, standard deviation is 92.8, 66% spots contain this read
|PRJNA170814||SRP014428||Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells|
We assessed Smart-Seq, a new single-cell RNA-Seq library preparation method, on a variety of mouse and human RNA samples or cells. Overall design: We generated RNA-Seq libraries for dilution series of MAQC reference RNA and mouse brain RNA to assess technical reproducibility, and for a variety of individual cells including putative circulating tumour cells.
You need SRA Toolkit to operate on SRA runs.
Default toolkit configuration enables it to find and retrieve SRA runs by accession. It also downloads (and cache) only the part of data you really need. For example quality scores represent a majority of data volume and you may not need them if you dump fasta only (versus fastq). Or if you are looking at particular gene you may not need reads aligned to other regions or not aligned at all. Same way if you use GATK with enabled SRA support you need only SRA run accessions to fire your process.
fastq-dump will dump reads in a number of "standard" fastq and fasta formats.
vdb-dump is also capable of producing fasta and fastq (beside other formats). It dumps data much faster then fastq-dump but ordering of reads may be different and it does not produce split-read multi-file output.
Prefetch tool will help you cache all data in advance if you plan to run data analysis in environment where getting data from NCBI at run time is unfeasible.
Read more at SRA Knowledge Base on how to download SRA data using command line utilities.
In addition to it you can download the following data:
|Type||Size||Name||Free Egress||Access Type|
The sections below show results of analysis run by software which is still in experimental stage. Please use provided results with a boatload of salt and let us know what you think.
-- SRA team
- Unidentified reads: 100%