Transcriptomic analysis on leaves of C. sinensis under low-pH
Background: Limited data are available on low-pH alterations of gene profiles in woody plants. Seedlings of low-pH insensitive Citrus sinensis and low-pH sensitive Citrus grandis were fertigated with nutrient solution at pH 2.5 and 6.0. Thereafter, we investigated the low-pH-induced alterations of transcriptomics in leaves by RNA-Seq. Results: Using RNA-seq, we isolated 231 upregulated and 351 downregulated genes from low-pH-treated C. grandis (C. sinensis) leaves. Conclusion:The inhibition of the seedling growth in low pH may be related to the differentially expression of genes in glycometabolism, amino acid metabolism, protein metabolism, carbon fixation in photosynthetic organisms, chloroplast component, porphyrin and chlorophyll metabolism and plant hormone signal transduction. Overall design: Examination of mRNA levels in control and low-pH-treatment leaves of C. sinensis with two-three biological replicates were generated by deep sequencing, using Illumina HiSeq xten device.
External Link: /pubmed:30208853