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Retinoic acid induced expression of Hnf1ß and Fzd4 is required for pancreas development in Xenopus laevis

Identifiers: SRA: SRP137258
BioProject: PRJNA448780
GEO: GSE112718
Study Type: 
Transcriptome Analysis
Abstract: Retinoic acid (RA) is required for pancreas specification in Xenopus and other vertebrates. However, the gene network that is directly induced by RA-signalling in this context remains to be defined. We identified the transcription factor Hnf1ß and the Wnt-receptor Fzd4/Fzd4s as direct RA-target genes through RNA-sequencing of in vitro generated pancreatic explants. Functional analyses of Hnf1ß and Fzd4/Fzd4s in programmed pancreatic explants and whole embryos revealed their requirement for pancreatic progenitor formation and differentiation. Hnf1ß and Fzd4/Fzd4s thus appear to be involved in pre-patterning events of the embryonic endoderm allowing for pancreas formation. Overall design: VegT/Noggin/Cyp26a1 programmed animal cap explants from Xenopus laevis, untreated and treated with RA/CHX and collected 2 hours after RA addition at the equivalent of gastrula stage 11; two replicates A and B for each condition
Center Project: GSE112718
External Link: /pubmed:29769220

Related SRA data

Experiments:
8 ( 8 samples )
Runs:
8 (9.6Gbp; 5.9Gb)
Additional objects:
File type count
fastq 8