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RNAseq for peach weeping and standard lateral shoot (branch) tips

Identifiers: SRA: SRP136085
BioProject: PRJNA439260
GEO: GSE112080
Study Type: 
Transcriptome Analysis
Abstract: Plant shoots typically grow upward in opposition to the pull of gravity. However, exceptions exist throughout the plant kingdom. Most conspicuous are trees with weeping or pendulous branches. While such trees have long been cultivated and appreciated for their ornamental value, the molecular basis behind the weeping habit is not known. Here, we characterized a weeping tree phenotype in Prunus persica (peach) and identified the underlying genetic mutation using a genomic sequencing approach. Weeping peach tree shoots exhibited a downward elliptical growth pattern and did not exhibit an upward bending in response to 90° reorientation. The causative allele was found to be an uncharacterized gene, Ppa013325, having a 1.8-Kb deletion spanning the 5' end. This gene, dubbed WEEP, was predominantly expressed in phloem tissues and encodes a highly conserved 129-amino acid protein containing a sterile alpha motif (SAM) domain. Silencing WEEP in the related tree species Prunus domestica (plum) resulted in more outward, downward, and wandering shoot orientations compared to standard trees, supporting a role for WEEP in directing lateral shoot growth in trees. This previously unknown regulator of branch orientation, which may also be a regulator of gravity per- ception or response, provides insights into our understanding of how tree branches grow in opposition to gravity and could serve as a critical target for manipulating tree architecture for improved tree shape in agricultural and horticulture applications. Overall design: mRNA was isolated from four shoot tips (approximately 4cm long) from standard and weeping peach trees. Four biological replicates for each type of tree were sequenced using illumina Hiseq2000
Center Project: GSE112080
External Link: /pubmed:29712856

Related SRA data

Experiments:
8 ( 8 samples )
Runs:
8 (9.8Gbp; 5.8Gb)
Additional objects:
File type count
fastq 8