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Identifying the genome-wide genetic variation between precocious trifoliate orange and its wild type and developing new markers for genetics research

Identifiers: SRA: SRP071050
BioProject: PRJNA314020
GEO: GSE78810
Study Type: 
Transcriptome Analysis
Abstract: To increase our understanding of the genes involved in flowering in citrus, we performed genome resequencing of an early flowering trifoliate orange mutant (Poncirus trifoliata L. Raf.) and its wild type. At the genome level, 3,932,628 single nucleotide polymorphisms (SNPs), 1,293,383 insertion/deletion polymorphisms (InDels), and 52,135 structural variations (SVs) were identified between the mutant and its wild type based on the citrus reference genome. Based on integrative analysis of resequencing and transcriptome analysis, 233,998 SNPs and 75,836 InDels were also identified between the mutant and its wild type at the transcriptional level. Also, 272 citrus homologous flowering-time transcripts containing genetic variation were also identified. GO and KEGG annotation revealed that the transcripts containing the mutant and the wild-type-specific InDel were involved in diverse biological processes and molecular function. Among these transcripts, there were 131 transcripts that were expressed differently in the two genotypes. When 268 selected InDels were tested on 32 genotypes of the three generas of Rutaceae for the genetic diversity assessment, these InDel-based markers showed high transferability. This work provides important information that will allow a better understanding of the citrus genome and that will be helpful for dissecting the genetic basis of important traits in citrus. Overall design: Two samples, the terminal bud and the five following buds (the major node position for flower formation) from spring flushes of the MT and the WT 2-year-old trees were collected at flower initiation (after self-pruning). And then subjected to RNA-seq
Center Project: GSE78810
External Link: /pubmed:27106267

Related SRA data

Experiments:
2 ( 2 samples )
Runs:
2 (2.4Gbp; 911.8Mb)
Additional objects:
File type count
fastq 4