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Single-cell RNA-seq reveals distinct maturation stages of the Paneth cell lineage

Identifiers: SRA: SRP067892
BioProject: PRJNA307195
GEO: GSE76408
Study Type: 
Transcriptome Analysis
Abstract: Paneth cells (PCs) are long-lived secretory cells that reside at the bottoms of small intestinal crypts. Besides serving as niche cells for the neighboring Lgr5-positive stem cells, PCs secrete granules containing a broad spectrum of antimicrobial proteins, including lysozymes and defensins1. Here, we have used single-cell RNA sequencing to explore PC differentiation. We found a maturation gradient from early secretory progenitors to mature PCs, capturing the full maturation path of PCs. Moreover, differential expression of a subset of defensin genes in lysozyme-high PCs, e.g. Defa20, reveals at least two distinct stages of maturation. Overall design: We traced Lgr5+ stem cells from Lgr5-CreERT2 C57Bl6/J mice bred to a Rosa26LSL-YFP reporter mice and sorted YFP+ cells 5 days, 3 weeks and 8 weeks after tamoxifen injection.
Center Project: GSE76408
External Link: /pubmed:27345837

Related SRA data

Experiments:
13 ( 13 samples )
Runs:
13 (33.1Gbp; 17.9Gb)