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Pol IV and RDR2-dependent precursors of 24 nt siRNAs

Identifiers: SRA: SRP059814
BioProject: PRJNA287854
Study Type: 
Abstract: In Arabidopsis thaliana, 24 nt siRNAs guide cytosine methylation to complementary DNA sequences, helping silence transposons and a subset of genes. Biogenesis of 24 nt siRNAs requires nuclear multisubunit RNA Polymerase IV (Pol IV), RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) and DICER-LIKE3 (DCL3). We used Illumina deep sequencing of 15-80 nt RNAs from wild type (Col-0) and dcl2 dcl3 dcl4 triple mutant plants to identify short dsRNAs that are synthesized by the Pol IV-RDR2 complex, and which are subsequently diced by DCL3.

Related SRA data

8 ( 4 samples )
8 (7.6Gbp; 5.4Gb)
Additional objects:
File type count
fastq 8