NCBI SRA Toolkit

Below are the latest releases of various tools and release checksum file.

SRA Toolkit

Compiled binaries/install scripts of Ferbuary 10, 2022, version 3.0.0:

• CentOS Linux 64 bit architecture - non-sudo tar archive
• Ubuntu Linux 64 bit architecture - non-sudo tar archive
• Cloud - apt-get install script - for Debian and Ubuntu - requires sudo permissions
• Cloud - yum install script - for for CentOS - requires sudo permissions
• MacOS 64 bit architecture
• MS Windows 64 bit architecture
• Docker image repository
• md5 checksums

Magic-BLAST

Magic-BLAST is a tool for mapping large next-generation RNA or DNA sequencing runs against a whole genome or transcriptome.

• Magic-BLAST executables for LINUX, MacOSX, and Windows as well as the source files are available on the FTP site
• Read more about Magic BLAST on the FTP site

Third Party Software

Builds of Third Party Software Tools with SRA support:

• HISAT2 version 2.2.1-ngs.3.0.0 - graph-based alignment of next generation sequencing reads to a population of genomes with direct support of SRA, built for:
  • CentOS Linux 64 bit architecture
  • MacOS 64 bit architecture

Latest Source Code

• NGS Software Development Kit – October 7, 2021, version 2.11.2 release
• NCBI VDB Software Development Kit – October 7, 2021, version 2.11.2 release
• NCBI SRA Toolkit – Ferbuary 10, 2022, version 3.0.0 release
• NCBI NGS Toolkit – October 7, 2021, version 2.11.2 release

File checksums

You may validate downloaded files with md5 checksums computed using md5sum -b