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Weak exons are hotspots for disruptive splicing perturbations

Identifiers: SRA: SRP231154
BioProject: PRJNA590968
GEO: GSE140786
Study Type: 
Transcriptome Analysis
Abstract: RNA-seq of amiloride-treated cell culture was conducted in order to understand the characteristics that make certain exons susceptible to splicing perturbations. Amiloride is a diuretic that acts by inhibiting epithelial sodium channels in the kidney. It also behaves as a non-specific kinase inhibitor, and has been shown to affect splicing in a handful of genes, presumably by altering phosphorylation of splicing factors. Two samples were sequenced (one untreated, one treated). Differential splicing was done using rMATS version 3.2.5. Overall design: Poly-A+ RNA from HEK293T cells untreated or treated with amiloride (300 µg/mL) for 6 h was isolated and prepared for RNA-seq.

Related SRA data

2 ( 2 samples )
2 (66.8Gbp; 21.7Gb)
Additional objects:
File type count
fastq 4