Single-cell RNA-seq analysis of human tonsil CD14+ cells
We performed single-cell RNA-seq on CD14+ cells isolated from the tonsils of one healthy donor. We used the 10x chromium technology. Overall design: Tonsil phagocytes were prepared by centrifugation on a Ficoll gradient. Dendritic cells and macrophages were enriched by negative selection using magnetic beads. Cell populations were further isolated by cell sorting. Cellular suspensions (3500 cells) were loaded on a 10X Chromium instrument (10X Genomics) according to manufacturer's protocol.
External Link: /pubmed:31072818
|Homo sapiens||SAMN09979284||tonsil_macrophages.bam||20.0Gb||2018-09-05 15:33:43|