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Single-cell RNA-seq analysis of human tonsil CD14+ cells

Identifiers: SRA: SRP159650
BioProject: PRJNA489435
GEO: GSE119506
Study Type: 
Other
Abstract: We performed single-cell RNA-seq on CD14+ cells isolated from the tonsils of one healthy donor. We used the 10x chromium technology. Overall design: Tonsil phagocytes were prepared by centrifugation on a Ficoll gradient. Dendritic cells and macrophages were enriched by negative selection using magnetic beads. Cell populations were further isolated by cell sorting. Cellular suspensions (3500 cells) were loaded on a 10X Chromium instrument (10X Genomics) according to manufacturer's protocol.
Center Project: GSE119506
External Link: /pubmed:31072818

Related SRA data

Experiments:
1 ( 1 samples )
Runs:
1 (19.5Gbp; 9.3Gb)
Additional objects:
File type count
10X Genomics bam file 1