Bolitoglossa ramosi differential expressed genes during limb regeneration
Tissue regeneration is widely distributed across the tree of life. Among vertebrates, salamanders possess an exceptional ability to regenerate amputated limbs and other complex structures. Thus far, molecular insights about limb regeneration have come from a relatively limited number of species from two closely related salamander families. To gain broader perspective on the molecular basis of limb regeneration and enhance the molecular toolkit of an emerging plethodontid salamander (Bolitoglossa ramosi), we used RNA-seq to generate transcript sequence data and identify 602 genes that are differentially expressed during limb regeneration. This list was further processed to identify a core set of genes that exhibit conserved expression changes between B. ramosi and the Mexican axolotl (Ambystoma mexicanum), and presumably their common ancestor approximately 180 million years ago. Our study highlights the importance of developing comparative gene expression data for studies of limb regeneration among salamanders. Overall design: To assembly the reference transcriptome, we sequenced individual samples which had pools of different adult animals we did pools of different animals to get the tissues of controls (three biological replicates, n = 9 animals), gut (one biological replicate, n = 1 animal), skin (one biological replicate, n = 1 animal), blastema 20 dpa (one biological, replicate n = 1 animal), blastema 40 dpa (one biological replicate, n = 2 animals), blastema 60 dpa (two biological replicates, n = 5 animals) and 70 dpa (one biological replicate, n = 3 animals).
External Link: /pubmed:30253734