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Enrichment of organohalide respiring bacteria

Identifiers: SRA: SRP091882
BioProject: PRJNA349488
Study Type: 
Metagenomics
Abstract: Organohalide respiring bacteria are difficult to enrich and isolate, which can limit research on these important organisms. The goal of this research was to develop a method to rapidly (minutes to days) enrich these organisms from a mixed culture or sediment sample. The method presented herein was based on the hypothesis that organohalide respiring bacteria would be more hydrophobic than other bacteria as a result of their niche dehalogenating hydrophobic compounds. To test this hypothesis, a method was developed to separate putative organohalide respiring bacteria at the interface between a hydrophobic organic solvent and aqueous media. This novel separation technique was tested with a sediment-free culture, a tetrachloroethene-enriched digester culture, and sediment from an uncontaminated lake.

Related SRA data

Experiments:
2 ( 2 samples )
Runs:
8 (312.2Mbp; 156.5Mb)
Additional objects:
File type count
fastq 16
assembled contigs 8