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Transcriptomics analysis of developing wild type and val1 mutant Arabidopsis embryos

Identifiers: SRA: SRP065807
BioProject: PRJNA301162
GEO: GSE74692
Study Type: 
Transcriptome Analysis
Abstract: Developing Arabidopsis seeds accumulate oils and seed storage proteins synthesized by the pathways of primary metabolism. Seed development and metabolism are positively regulated by transcription factors belonging to the LAFL regulatory network. The VAL gene family encodes repressors of the seed maturation program in germinating seeds, although they are also expressed during seed maturation. VAL1 functions as a repressor of seed metabolism, as val1 mutant seeds accumulated elevated levels of storage proteins compared to the wild type. Two VAL1 splice variants were identified through RNA sequencing analysis: a full-length and a truncated form lacking the plant-homeodomain-like domain associated with epigenetic repression. None of the transcripts encoding the core LAFL network transcription factors were affected in val1 embryos. Instead, activation of VAL1 by FUSCA3 appears to result in repression of a subset of seed maturation genes downstream of core LAFL regulators as 39% of transcripts in the FUSCA3 regulon were de-repressed in the val1 mutant. The LEC1 and LEC2 regulons also responded but to a lesser extent. Additional 832 transcripts that were not LAFL targets were de-repressed in val1 mutant embryos. These transcripts are candidate targets of VAL1, acting through epigenetic and/or transcriptional repression. Overall design: 2 genotypes, 7 time points, 3 biological and 4 technical replicates
Center Project: GSE74692
External Link: /pubmed:26678037

Related SRA data

Experiments:
41 ( 41 samples )
Runs:
41 (185.4Gbp; 98.8Gb)
Additional objects:
File type count
fastq 82