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Effect of OVO-like 1 knockdown on global transcript expression in differentiated BeWo trophoblast cells

Identifiers: SRA: SRP056220
BioProject: PRJNA278477
GEO: GSE66962
Study Type: 
Transcriptome Analysis
Abstract: We had previously discovered that the transcription factor OVO-like 1 (OVOL1) was highly induced during trophoblast differentiation. In this study, we used an lentiviral shRNA strategy to decrease OVOL1 expression in BeWo trophoblast cells. Control cells were transduced with shRNAs targeting no known mammalian transcript (shCont). Following stimulation of differentiation (48h exposure to 8-bromo-cyclic adenosine monophosphate), a RNA-seq approach was used to determine global transcript differences in OVOL1-knockdown cells compared to control cells. Overall design: Trophoblast cells transduced with control shRNAs were used as controls. Cells transduced with shRNAs targeting OVOL1 were used as treatment. All cells received 250 uM 8-bromo-cyclic adenosine monophosphate to stimulate differentiation. Three independent replicates of control and treatment groups were analyzed.
Center Project: GSE66962
External Link: /pubmed:26504231

Related SRA data

6 ( 6 samples )
43 (31.6Gbp; 20.4Gb)