Intrinsic age-dependent changes and cell-cell contacts regulate nephron progenitor lifespan
Study Type:
Transcriptome Analysis
Abstract: Purpose: To use single-cell RNA-Seq analysis of nephron progenitors in order to determine transcrptional differences as nephron progenitors age. Methods: Using a combination of FACS sorting and a Fluidigm Single-cell auto-prep system, we generated high-throughput RNA-SEQ data of nephron progenitors during development Results: Single cells transcriptome profiling of nephron progenitors revealed progressive age-dependent changes with heterogeneity increasing in older populations. Overall design: 96-single cell transcriptomes were determined from nephron progenitors of e14.5, e18.5 and P0 using Cited1GFP transgenic animals
Center Project: GSE66202
External Link: /pubmed:26460946
| Organism | Sample | File Name | Size | Updated |
|---|---|---|---|---|
| Mus musculus | SAMN03366442 | SRR1813568.pileup | 6.6Mb | 2016-02-10 00:48:23 |
| Mus musculus | SAMN03366426 | SRR1813569.pileup | 6.0Mb | 2016-02-10 00:50:53 |
| Mus musculus | SAMN03366497 | SRR1813575.pileup | 6.2Mb | 2016-02-10 00:47:57 |
| Mus musculus | SAMN03366446 | SRR1813579.pileup | 6.6Mb | 2016-02-10 00:47:17 |
| Mus musculus | SAMN03366422 | SRR1813580.pileup | 5.1Mb | 2016-02-10 00:44:53 |
| Mus musculus | SAMN03366434 | SRR1813582.pileup | 4.9Mb | 2016-02-10 00:44:12 |
| Mus musculus | SAMN03366502 | SRR1813584.pileup | 5.9Mb | 2016-02-10 00:44:19 |
| Mus musculus | SAMN03366438 | SRR1813586.pileup | 6.2Mb | 2016-02-10 00:44:39 |
| Mus musculus | SAMN03366433 | SRR1813590.pileup | 6.0Mb | 2016-02-10 00:42:58 |
| Mus musculus | SAMN03366423 | SRR1813595.pileup | 6.0Mb | 2016-02-10 00:44:10 |
| Mus musculus | SAMN03366454 | SRR1813596.pileup | 6.0Mb | 2016-02-10 00:41:23 |
| Mus musculus | SAMN03366455 | SRR1813597.pileup | 5.7Mb | 2016-02-10 00:43:52 |
| Mus musculus | SAMN03366468 | SRR1813598.pileup | 5.2Mb | 2016-02-10 00:42:40 |
| Mus musculus | SAMN03366427 | SRR1813599.pileup | 5.9Mb | 2016-02-10 00:47:01 |
| Mus musculus | SAMN03366484 | SRR1813605.pileup | 5.6Mb | 2016-02-10 00:42:13 |
| Mus musculus | SAMN03366492 | SRR1813609.pileup | 6.3Mb | 2016-02-10 00:39:30 |
| Mus musculus | SAMN03366429 | SRR1813611.pileup | 5.9Mb | 2016-02-10 00:39:39 |
| Mus musculus | SAMN03366470 | SRR1813614.pileup | 4.9Mb | 2016-02-10 00:47:26 |
| Mus musculus | SAMN03366420 | SRR1813621.pileup | 5.2Mb | 2016-02-10 00:46:49 |
| Mus musculus | SAMN03366487 | SRR1813626.pileup | 6.2Mb | 2016-02-10 00:39:48 |
| Mus musculus | SAMN03366496 | SRR1813627.pileup | 5.5Mb | 2016-02-10 00:39:09 |
| Mus musculus | SAMN03366482 | SRR1813629.pileup | 5.7Mb | 2016-02-10 00:39:21 |
| Mus musculus | SAMN03366413 | SRR1813631.pileup | 4.9Mb | 2016-02-10 00:37:59 |
| Mus musculus | SAMN03366489 | SRR1813632.pileup | 5.4Mb | 2016-02-10 00:37:27 |
| Mus musculus | SAMN03366459 | SRR1813641.pileup | 5.9Mb | 2016-02-10 00:30:53 |
| Mus musculus | SAMN03366451 | SRR1813643.pileup | 5.8Mb | 2016-02-10 00:30:54 |
| Mus musculus | SAMN03366481 | SRR1813645.pileup | 5.5Mb | 2016-02-10 00:32:58 |
| Mus musculus | SAMN03366440 | SRR1813647.pileup | 6.2Mb | 2016-02-10 00:30:53 |
| Mus musculus | SAMN03366480 | SRR1813651.pileup | 4.0Mb | 2016-02-10 00:30:29 |
| Mus musculus | SAMN03366449 | SRR1813652.pileup | 4.6Mb | 2016-02-10 00:32:02 |