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Transcriptome profiling in human T-ALL

Identifiers: SRA: SRP042286
BioProject: PRJNA248647
GEO: GSE57982
Study Type: 
Transcriptome Analysis
Abstract: Genome-wide mapping and characterization of novel Notch-regulated long non-coding RNAs in acute leukemia Overall design: Total RNA was extracted from samples using the RNeasy Plus mini kit (Life Technologies, Carlsbad, CA). Samples were then subject to PolyA selection (Figures 1E, 5F and 5G only) using oligo-dT beads (Life Technologies, Carlsbad, CA) or rRNA removal (all other samples) using the Ribo-Zero kit (Epicentre, Madison, WI) according to the manufacturers instructions. The resulting RNA samples were then used as input for library construction using the dUTP method as described by Parkhomchuck et al, 2009. RNA libraries were then sequenced on the Illumina HiSeq 2000 or 2500 using 50bp paired-end reads.
Center Project: GSE57982
External Link: /pubmed:25083870

Related SRA data

Experiments:
31 ( 31 samples )
Runs:
55 (174.6Gbp; 101.1Gb)