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Variation in RNA-Seq transcriptome profiles of peripheral whole blood from healthy individuals with and without globin depletion

Identifiers: SRA: SRP034732
BioProject: PRJNA232593
GEO: GSE53655
Study Type: 
Transcriptome Analysis
Abstract: In this study we aimed to assess technical variability associated with globin depletion in addition to assessing general technical variability in RNA-Seq from whole blood derived samples. We compared technical and biological replicates having undergone globin depletion or not and found that globin depletion removed approximately 80% of globin transcripts, improved the correlation of technical replicates, allowed for reliable detection of thousands of additional transcripts and generally increased transcript abundance measures. Overall design: Peripheral whole blood transcriptome assessed by RNA-Seq on Illumina HiSeq 2000 in 6 healthy individuals and 6 pooled samples, either globin depleted or not.
Center Project: GSE53655
External Link: /pubmed:24608128

Related SRA data

Experiments:
47 ( 47 samples )
Runs:
47 (138.8Gbp; 87.2Gb)