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Evolutionary dynamics of gene and isoform regulation in mammalian tissues

Identifiers: SRA: SRP016501
BioProject: PRJNA177791
GEO: GSE41637
Study Type: 
Abstract: Most mammalian genes produce multiple distinct mRNAs through alternative splicing, but the extent of splicing conservation is not clear.  To assess tissue-specific transcriptome variation across mammals, we sequenced cDNA from 9 tissues from 4 mammals and one bird in biological triplicate, at unprecedented depth.  We find that while tissue-specific gene expression programs are largely conserved, alternative splicing is well conserved in only a subset of tissues and is frequently lineage-specific. Thousands of novel, lineage-specific and conserved alternative exons were identified; widely conserved alternative exons had signatures of binding by MBNL, PTB, RBFOX, STAR and TIA family splicing factors, implicating them as ancestral mammalian splicing regulators.  Our data also indicates that alternative splicing is often used to alter protein phosphorylatability, delimiting the scope of kinase signaling. Overall design: Tissue transcriptomes from 9 tissues from 5 species, 3 individuals per species, were sequenced and compared (two samples for mouse_heart)
Center Project: GSE41637
External Link: /pubmed:23258891

Related SRA data

134 ( 134 samples )
134 (1.1Tbp; 659.6Gb)
Additional objects:
File type count
fastq 77