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GSE39128: Saccharomyces cerevisiae Transcript Isoform mapping

Identifiers: SRA: SRP014137
GEO: GSE39128
Study Type: 
Transcriptome Analysis
Abstract: Summary: Extensive transcriptional heterogeneity revealed by isoform profiling Overall Design: Application of TIF-Seq (Transcript IsoForm Sequencing) to S.cerevisiae. The method was applied to simultaneously identify the 5' capped mRNA transcription start site and the 3' polyadenylation site in different conditions: WT cells grown in glucose media [ypd, 2 biological replicates (bio) and 3 independent library preparations, technical replicates(lib)], WT cells grown in galactose media [ypgal, 4 biological replicates (bio) and 3 independent library preparations, technical replicates(lib)]. A modified protocol designed to enrich in long mRNA molecules was performed for WT cells grown in glucose media [ypd, 2 biological replicates (bio)] and in galactose media [ypgal, 2 biological replicates (bio)] conditions. Finally, control samples performed with a modified protocol designed to identify non-capped but polyadenylated molecules was performed in WT cells grown both in glucose (nypd) and galactose (nypgal) media.
Center Project: GSE39128: Saccharomyces cerevisiae Transcript Isoform mapping
External Links: GEO Series
/gds:200039128

Related SRA data

Experiments:
18
Runs:
26 (123.1Gbp; 73.4Gb)