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Human Microbiome Project (HMP) 16S rRNA Gene Diversity, the diversity of 16S ribosomal RNA genes in the human microbiome: 454 Protocol Validation - Mock

Identifiers: SRA: SRP002397
BioProject: PRJNA48341
HMP-DACC: hmp_mock_pilot_CEFoS_study
Study Type: 
Metagenomics
Abstract: This HMP Centers'' Evaluation of the standard 454 SOP represents the pyrosequencing of 16S rRNA genes amplified from HMP even Mock community distributed to each of the four HMP sequencing centers. The goal of the pilot was to test the provisional 454 sequencing protocol (v4.2) and to evaluate accuracy. This protocol does use barcodes, targets two 16S windows (V1-V3 and V3-V5), and sequences in the reverse direction. (Previously referred to as Centers'' Evaluation of 454 SOP (CEFoS) Mock Pilot)
Center Project: human metagenome
External Link: NIH HMP DACC

The SRA runs have been pre-filtered by NCBI to remove contaminating human sequence.

These data are available through the dbGaP authorized access system.


Related SRA data

Experiments:
18 ( 3 samples )
Runs:
133 (25.7Gbp; 15.8Gb)