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"ChIP-seq of PolII unmodified CTD and Ser5ph in mESC Wild Type and Setd5 Hetero and Homo"

Identifiers: SRA: ERP110680
BioProject: PRJEB28473
EUROPEAN MOLECUALR BIOLOGY LABORATORY, GENOME BIOLOGY UNIT: ChIP-seq_of_PolII_unmodified_CTD_and_Ser5ph_in_mESC_Wild_Type_and_Setd5_Hetero_and_Homo_expid_600
Study Type: 
Other
Abstract: ChIP-seq for RNA Polymerase II with an antibody recognising unmodified CTD and Serine 5 Phosphorylation CTD (clone 4H8). For Each replicate of 100 million ESCs were harvested and cross linked in 1.5mM EGS and 1.5% PFA digested with MNase and mildly sonicated to achieve mono-di-tri nucleosome fragmented chromatin. Antibody: CST 2629, Lot No 3 ref 10/2017
Description: ChIP-seq for RNA Polymerase II with an antibody recognising unmodified CTD and Serine 5 Phosphorylation CTD (clone 4H8). For Each replicate of 100 million ESCs were harvested and cross linked in 1.5mM EGS and 1.5% PFA digested with MNase and mildly sonicated to achieve mono-di-tri nucleosome fragmented chromatin. Antibody: CST 2629, Lot No 3 ref 10/2017

Related SRA data

Experiments:
9 ( 9 samples )
Runs:
9 (10.6Gbp; 7.2Gb)