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Metagenome from Paralichthys olivaceus

Identifiers: SRA: DRP000303
BioProject: PRJDA53873
NIID: DRP000303
Study Type: 
Metagenomics
Abstract: Food-poisoning fillet of Paralichthys olivaceus. Total DNA or RNA was prepared from a 30 mg of the frozen fillet of Paralichthys olivaceus by RecoverAll Total Nucleic Acid isolation kit (Ambion, Austin, TX USA). Double-stranded cDNA was prepared from 1 micro g of total RNA using the random priming method with SuperScript Choice System for cDNA synthesis (Invitrogen, Carlsbad, CA, USA). cDNA was purified using a QIAquick PCR Purification kit (QIAGEN, Hilden, Germany). Short-read DNA sequencing using the Illumina Genome Analyzer II An approximately 100-bp length DNA library was prepared from the mixture of 2 micro g of total DNA and 0.5 micro g of cDNA using a genomic DNA sample prep kit (Illumina, San Diego, CA, USA), and DNA clusters were generated on a slide using a Single Read Cluster Generation kit (ver. 2) on an Illumina cluster station (Illumina), according to the manufacturer's instructions.
Center Project: food metagenome
External Link: /pubmed:22281845

Related SRA data

Experiments:
2 ( 2 samples )
Runs:
2 (2.4Gbp; 1.8Gb)