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Comprehensive analysis of polysomal mRNAs in HT29 cell at 16hr after treatment with tunicamycin.

Identifiers: SRA: DRP000016
BioProject: PRJDA34559
UT-MGS: DRP000016
Study Type: 
Transcriptome Analysis
Submission: DRA000016
Abstract: Expression profile in HT29 cell exposed to ER stress was attempted. We used shotgun sequencing method, in which next gene sequencing technology and polysome analysis were combined.
Description: We analyzed the mRNA profile in cytoplasm and in polysome during the endoplasmic reticulum (ER) stress using second generation sequencers. We compared three time points (0 hr/untreatment control, 4 hr and 16 hr after treatment with 2 ??g/mL ER stress inducible agent Tunicamycin (Tm)). This is the first time mRNA in ???polysome??? is analyzed using the next generation sequencers and compared with that of cytoplasm.
Center Project: Integrateve Transcriptome Analysis
External Link: /pubmed:22465528

Related SRA data

Experiments:
1
Runs:
1 (147.3Mbp; 694.8Mb)