Comprehensive analysis of polysomal mRNAs in HT29 cell.
Study Type:
Transcriptome Analysis
Submission: DRA000014
Abstract: Expression profile in HT29 cell exposed to ER stress was attempted. We used shotgun sequencing method, in which next gene sequencing technology and polysome analysis were combined.
Description: We analyzed the mRNA profile in cytoplasm and in polysome during the endoplasmic reticulum (ER) stress using second generation sequencers. We compared three time points (0 hr/untreatment control, 4 hr and 16 hr after treatment with 2 ??g/mL ER stress inducible agent Tunicamycin (Tm)). This is the first time mRNA in ???polysome??? is analyzed using the next generation sequencers and compared with that of cytoplasm.
Center Project: Integrateve Transcriptome Analysis
External Link: /pubmed:22465528