Comprehensive analysis of cytoplasmic mRNAs in HT29 cell at 16hr after treatment with tunicamycin.
Identifiers: SRA: DRP000013
Expression profile in HT29 cell exposed to ER stress was attempted. We used shotgun sequencing method, in which next gene sequencing technology and polysome analysis were combined.
We analyzed the mRNA profile in cytoplasm and in polysome during the endoplasmic reticulum (ER) stress using second generation sequencers. We compared three time points (0 hr/untreatment control, 4 hr and 16 hr after treatment with 2 ??g/mL ER stress inducible agent Tunicamycin (Tm)). This is the first time mRNA in ???polysome??? is analyzed using the next generation sequencers and compared with that of cytoplasm.
Integrateve Transcriptome Analysis
External Link: /pubmed:22465528