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Subsurface mine microbial mat metagenome

Identifiers: SRA: DRP000009
BioProject: PRJNA39577
JAMSTEC: DRP000009
Study Type: 
Metagenomics
Abstract: This is a metagenomic project to figure out the microbial community flourished along with hot water stream in the Japanese subsurface mine.
Description: This is a metagenomic project to figure out the microbial community flourished along with hot water stream in the Japanese subsurface mine. As a first step of this project, we constructed a fosmid library from the DNA directly isolated from the microbial mat without cultivation. Since we could get about 3,500 fosmid clones, we determined the paired-end sequence of all fosmid clones. Next, we classified all clones in phylum level based on the information for the origin of the genes identified in the paired-end sequence and then we selected 179 clones evenly according to the ratio of each classified phylum to cover whole microbial community. These selected clones were sequenced by shotgun sequencing method (Sanger). Moreover, we selected 158 fosmid clones to increase the genomic information in the microbial mat and sequenced all the selected clones at once by 454 sequencer.
External Link: /pubmed:22303444

Related SRA data

Experiments:
1 ( 1 samples )
Runs:
3 (259.6Mbp; 608.2Mb)