Comprehensive identification and characterization of the transcripts, their expression levels and sub-cellular localizations
Identifiers: SRA: DRP000005
Comprehensive identification and characterization of the transcripts, their expression levels and sub-cellular localizations in mammalian genes were attempted. We used RNA-Seq method, in which next gene sequencing technology and shotgun sequencing of the RNAs were combined. We prepared RNA Seq libraries from several sub-cellular fractions and carried out the massive sequencing.
Although recent studies have revealed that the majority of human genes are subjected to regulation of alternative promoters (APs), the biological relevance of this phenomenon remains unclear. In order to understand biological significance of the presence of divergent transcription initiation events in the respective cell types, it is indispensable to obtain bird-view of the transcriptome figures at every step of the gene expression; namely, i) how the genomic structure change to transcriptionally active form, ii) where the transcription initiation complex is recruited, iii) to what extent the transcription is activated, iv) what transcripts formed and sorted to what subcellular fractions. We have recently started multi-faceted use of the Illumina GA to answer these questions. Integrative analysis produced for respective aspects of the gene expression regulations revealed the comprehensive figures of the complex human gene transcriptome for the first time.
Integrative Transcriptome Analysis