|Run||Spots||Bases||Size||GC content||Published||Access Type|
This run has 2 reads per spot:
|L=90, 100%||L=90, 100%|
Technical read Application Read L=4, 100% Length is 4, 100% spots contain this read ̅L=165, σ=92.8, 66% Average length is 165, standard deviation is 92.8, 66% spots contain this read
|SAMN01801655 (SRS374188)||The ray florets in five sample pools from early p1 and P1, P2 to P5 to constitute our cDNA library for the transcriptome in the gerbera flower growth. All fresh samples collected were snap-frozen immediately in nitrogen and stored at -80℃ to extract the total RNA.Total RNA was extracted using the TRIZol reagent and an isolation system according to the manufacturer’s protocol and treated with DNase. To avoid losing low expression transcripts during early envelopment, a pooled RNA sample with early P1 and P1, P2, P3, P4 and P5 was mixed in a ratio of 2:1.25:1:1:1 after measuring the integrity.||Gerbera hybrida|
|PRJNA179026||SRP017069||Gerbera hybrid cultivar strain:Shenzhen No.5 Transcriptome or Gene expression|
SRA Toolkit tools directly operate on SRA runs. Toolkit has capacity to find requested runs at NCBI and download (and cache) only the part you really need. For example quality scores represent a majority of data volume and you may not need them if you dump fasta only (versus fastq). Or if you are looking at particular gene you may not need the reads aligned to other regions or not aligned at all.
If you plan to run data analysis in environment where getting data from NCBI at run time is unfeasible you can use SRA Toolkit prefetch utility to cache all data in advance. Read more at SRA Knowledge Base on how to download SRA data using command line utilities.
Please use SRA Run Selector to filter and download list of SRA runs in the scope of experiment, sample or study.